FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2588_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2588_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2965060
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA17600.054.3797451
GCGAAAG19500.050.9403422
GAGCGAA10550.046.7339361
CGAAAGC22300.045.1945273
GAGCAAA54800.041.016351
CAAAAGC58400.038.860414
GCAAAAG58850.037.9472353
AGCAAAA74200.029.7060952
AAAAGCA105300.022.1030675
GCTATAT12600.021.8669851
AAAGCAG127100.021.5065986
GTATATA22750.020.7160931
TAATACG4455.5442797E-817.922914
AAGCAGG178200.016.0292477
TATATAC22850.015.8657173
GTATTAA22900.015.1978321
TCTATAC20150.015.112983
CTAGTAC4902.968187E-614.7972093
GAAAGCA71800.014.6426434
CTATAAC8008.731149E-1114.5012651
Produced by FastQC (version 0.11.5)