FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2588_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2588_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677067
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG25650.048.623582
CGAAAGC26900.046.0945633
AGCGAAA28250.043.6351361
GAGCAAA92600.042.7550621
GCAAAAG95450.041.0226483
CAAAAGC98800.040.145994
TTATCGC3850.033.90142
AGCAAAA115950.033.8323672
GTGATCG6900.033.6229559
TATCGCT4500.029.0045343
AAAGCAG162350.028.540426
AAAAGCA147000.026.9824755
GAGCGAA24550.026.2872031
GTATATA25800.025.57571
AAGCAGG199350.021.4567957
ATACCGT2205.9007324E-419.7760876
TCGTCGA2205.901273E-419.7758181
GCTATAT11850.019.581121
AGTACGG3001.1052201E-519.3366185
TATATAC29550.018.1585043
Produced by FastQC (version 0.11.5)