FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57678930
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG661710.11472300196969673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG142450.064.861975
CTATACG100200.063.624386
GCTATAC112100.063.227115
CTTATAG134500.063.0079545
GTATAGC153250.062.821336
GTATAGG276600.062.363056
TATAGGG259400.062.2278257
TATAGGC224900.061.9574057
TATACGG162950.061.7600367
GTATAGA188600.061.4928746
TATAAGG197300.061.3472027
AGTATAG229550.061.2513285
CTATAGG160250.061.036336
CATATAG193700.060.9917265
CGTATAA137000.060.7872625
TATAGTC180800.060.666637
TATAGCG181500.060.6058657
CTATAAG118250.060.323016
TTATACG143650.060.040446
GGTATAG236950.059.898655
Produced by FastQC (version 0.11.5)