FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-p1107-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-p1107-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6237064
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC123780.19845876200725215No Hit
GAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCT68680.11011591351315299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG60150.067.8101656
TTATAGC96150.067.269716
CCTATAC46850.067.049985
CTATAGG102000.067.0125666
GCTATAC59150.066.8866965
CTATACC53150.066.8027956
GGTATAC88400.066.598395
CGTATAG90550.066.5623555
CTTATAG86800.066.4960865
GTATAGG188200.066.4118046
GCTATAG104600.066.383585
TTATAGG172950.066.240356
TTATAAG114900.066.217246
GTATAAG133500.066.08396
GGTATAG155850.065.92185
CTATAAC59300.065.891646
GGTATAA113350.065.888285
CGTATAA78450.065.235385
CGTATAC59500.065.141045
TTTATAG139750.065.0308155
Produced by FastQC (version 0.11.5)