FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-p1107-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-p1107-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6431039
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC137640.21402451454578336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG60900.068.118056
CTATAGG110000.067.85736
CCTATAC46950.067.720965
CGTATAG98600.067.579185
CTATACC59400.067.306136
TTATAGC94700.067.2966166
GCTATAC66000.067.0411455
GGTATAC88300.067.024645
TTATAGG169050.066.893726
GGTATAA112500.066.784645
GTATAGG187800.066.26826
GGTATAG155250.066.170925
GCTATAG105900.066.091555
GTATAGC93550.065.992686
GTATAGA144400.065.813856
CTTATAG87100.065.739345
TTTATAG133500.065.659065
TTATAAG118800.065.569016
GTATAAG127800.065.467416
CATATAG133400.065.419855
Produced by FastQC (version 0.11.5)