FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-p1107-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-p1107-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8279873
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC188560.22773296160460435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG147050.067.881526
CTATACG80750.067.395316
TTATAGC123750.067.03976
CGTATAG121350.066.982135
GCTATAC85800.066.9342655
GGTATAA146350.066.772295
TTATAGG223450.066.710736
GTATAGG253750.066.642786
CTATACC75700.066.439396
GGTATAG206300.066.407475
GTATAAG166000.066.116146
CCTATAC63750.066.110355
CTTATAG113050.066.083545
GCTATAG142200.066.064645
TTATAAG150150.065.920996
CATATAG168000.065.891145
CGTATAA104400.065.8636255
CTATAAC75200.065.6253746
GTATAGA196200.065.601146
GGTATAC126650.065.5874945
Produced by FastQC (version 0.11.5)