FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-p1107-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-p1107-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6413239
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC152970.2385222194276558No Hit
GAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCT72310.1127511386991815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG106450.067.161866
CTATACG60200.067.1077966
GGTATAC90700.067.024475
TTATAGC94000.066.903576
GGTATAA116250.066.886345
CTATACC61400.066.821646
TTATAGG177250.066.718526
CGTATAG93750.066.597485
GTATAGG192050.066.348696
GCTATAG108900.066.293765
CCTATAC49750.066.158775
GCTATAC65400.066.158075
GTATAGC94900.066.0110866
GGTATAG156850.065.919035
CTATAAG80500.065.609786
CTTATAG85500.065.53715
TTATAAG125500.065.495526
CATATAG125550.065.414225
AGTATAG167650.065.386235
ATTATAG144700.065.290045
Produced by FastQC (version 0.11.5)