FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-p1107-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-p1107-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7850091
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC202250.25764032544336113No Hit
AATAAGTATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCC108800.13859711944740513No Hit
TAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCCTG100560.12810042584219725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG144650.068.1440666
CTATACG71750.068.007666
CGTATAG122700.067.534975
TTATAGC117500.067.308236
GTATAGG274050.066.996526
TTATAGG227600.066.9300846
GGTATAA142850.066.823865
GCTATAG143900.066.627955
CTTATAG108450.066.603765
GGTATAG220000.066.460235
GCTATAC78600.066.442435
TTATAAG151000.066.297666
CGTATAA97850.066.276135
CTATACC72800.066.258016
ATTATAG174750.066.1749955
CATATAG178200.066.1304555
CTATAAC60500.066.084016
GTATAAG168250.066.049216
GTATAGA213600.066.027486
GGTATAC127450.065.841345
Produced by FastQC (version 0.11.5)