FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-p1107-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-p1107-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6000114
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC113280.1887964128681555No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA61300.10216472553688147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG58450.067.261376
CGTATAA79900.067.152555
CTATAGG103700.067.019786
CGTATAG84300.067.008345
TTATAGC93000.066.907836
TTATAGG162550.066.6189656
GCTATAG99900.066.453015
GTATAGG178250.066.36336
CTATACC52450.066.286456
CCTATAC47050.066.0885245
GGTATAG147250.066.058645
GGTATAA112350.066.000415
TTATAAG105200.065.631956
GCTATAC62900.065.5612265
TTTATAG131750.065.36145
TATAGGG182100.065.304887
CTATAAC53050.065.273026
GTATAAG126500.065.198436
CTTATAG82400.065.15765
GTATAGC92400.065.0710456
Produced by FastQC (version 0.11.5)