FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-5421-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-5421-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4764361
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC94300.19792790680639016No Hit
GAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCT49940.10481993283044673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG86550.068.420466
CTTATAG65200.067.6493455
GGTATAG121850.067.60235
GTATAGG152650.067.5502246
CTATACG41750.067.5265666
TTATAGG128000.067.414816
GCTATAC45900.067.365215
GGTATAA86100.066.909315
GGTATAC70250.066.769575
CGTATAG67350.066.736235
TTATAGC72350.066.7170266
GCTATAG79800.066.71265
CTATACC46950.066.5291446
CCTATAC35650.066.2278065
TTATAAG91950.066.076076
TATAGGG134600.066.056797
CTATAAG60250.066.008456
GTATAGA113300.065.9119346
GTATAAG101250.065.63796
CTATAAC40450.065.546096
Produced by FastQC (version 0.11.5)