FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-5421-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-5421-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4458000
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC102940.23091072229699414No Hit
TAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCCTG59410.13326603858232391No Hit
AATAAGTATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCC47180.10583221175414985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG42550.067.65346
CTATAGG83300.067.603676
CTTATAG64650.067.412265
GTATAGG144400.067.411726
CGTATAG72050.067.236515
TTATAGG124150.067.025056
GGTATAG121300.066.95625
GCTATAC46350.066.634695
GGTATAC70800.066.4474645
CGTATAA58000.066.276435
CTATACC39050.066.193116
GGTATAA84350.066.1815955
GTATAGA118200.066.137766
CCTATAG52250.066.0724265
CATATAG105800.066.0540165
TTATAGC69100.066.006846
TATAGGG128650.065.8746647
AGTATAG126050.065.459775
TTTATAG92500.065.4552845
CTATAAC37300.065.453976
Produced by FastQC (version 0.11.5)