FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-5421-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-5421-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6063733
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC99250.1636780511279108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG57650.067.288246
CTATAGG105150.067.097076
TTATAGG168150.066.8999946
CTATACC61000.066.632066
CTTATAG83800.066.36825
GTATAGG188000.066.3297966
GGTATAA108850.066.101725
GGTATAG156550.066.047795
TATAGGG167350.065.65117
CTATAAG75300.065.637826
CGTATAG86150.065.613475
CTATAAC52250.065.539536
GGTATAC94450.065.4767765
GCTATAC62100.065.3391345
GTATAAG125950.065.264446
TTATAGC93350.065.236616
TATAAGG139250.065.1841357
GTATAGC98900.065.077146
CATATAG117750.064.9975
TCTATAG88800.064.955315
Produced by FastQC (version 0.11.5)