FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-5421-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-5421-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5140152
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC101370.1972120668805125No Hit
GAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCT66910.13017124785414905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG51700.067.381016
CTTATAG65300.067.273685
CTATAGG83950.067.243696
GGTATAG123950.067.1017765
TTATAGG137700.066.929166
GCTATAG82900.066.8718645
GTATAGG149300.066.836046
CGTATAG71300.066.714175
TTATAGC79300.066.643846
CTATACC47850.066.297036
GCTATAC51050.066.252075
GGTATAA90700.066.24985
GTATAGA110400.066.21346
CTATAAG66100.066.089086
TTTATAG111250.065.7703865
GGTATAC74400.065.6738055
TTATAAG96200.065.625236
TATAGGG145950.065.6135947
GTATACC69150.065.298436
GCTATAA68600.065.261125
Produced by FastQC (version 0.11.5)