FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-5421-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-5421-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4996807
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC99800.19972754601088252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG80450.067.221916
TTATAGG133350.067.075686
GCTATAC49550.066.642525
CTATACG50250.066.550186
CGTATAG73250.066.426685
CTTATAG68200.066.421475
TTATAGC73350.066.289096
GTATAGG147250.066.207796
GCTATAG79000.066.108265
CTATAAG64200.065.547786
TATAGGG139150.065.384667
GGTATAG119400.065.331645
CCTATAC39300.065.243085
GGTATAC74050.065.142175
GGTATAA85500.065.1328355
CGTATAA59300.065.064475
GTATAGA110900.065.0405966
TTATAAG90850.065.032756
CTATACC47100.064.9852146
CATATAG101850.064.705765
Produced by FastQC (version 0.11.5)