FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-5421-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-5421-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3788917
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC76450.2017726965251548No Hit
GAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCT45980.12135393834174779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG36350.067.450736
TTATAGG102600.067.3275456
GCTATAC39000.067.2619865
CTATAGG63100.067.180976
CTATACC36000.067.134936
CCTATAC28650.066.656285
CGTATAG54100.066.5259555
GCTATAG62300.066.190985
GTATAGG112300.066.1838766
GGTATAC56800.066.1346365
TTATAGC55800.065.752816
GGTATAG92700.065.688915
GGTATAA66800.065.658985
CTATAAG45750.065.594776
CTTATAG49500.065.57165
TATAGGG106250.065.342117
GTATAGA84900.065.214816
TTATAAG71650.064.924486
GCTATAA50450.064.891485
GTATAAG76150.064.854166
Produced by FastQC (version 0.11.5)