FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-5389-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-5389-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5421270
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC130460.2406447197796826No Hit
TAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCCTG63090.11637494535413288No Hit
AATAAGTATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCC59310.10940240939853578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG81150.067.2350855
CTATACC51350.067.22596
CTATAGG100950.067.213316
TTATAGG159050.067.025676
CTATACG48100.066.8963856
GGTATAC88700.066.835315
GCTATAC53750.066.826995
GTATAGG174450.066.782646
GGTATAG143200.066.701965
CTTATAG78000.066.497675
TTATAAG103450.066.332816
GGTATAA98200.066.317495
CGTATAA66500.066.268755
GCTATAG97400.066.036295
CATATAG123600.066.017065
CTATAAC43100.065.974066
GTATAGC87700.065.962316
TATAGGG160550.065.9413767
CTATAAG69650.065.882896
TTATAGC81900.065.850696
Produced by FastQC (version 0.11.5)