FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-5389-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-5389-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5149950
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC113170.21974970630782822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG82700.067.4690556
CTATACG49950.067.233426
CGTATAG76100.066.6550145
TTATAGC78550.066.401966
GGTATAA91450.066.368995
GTATAGG154600.066.3669746
CTTATAG67800.066.301435
CTATACC48750.066.233326
TTATAGG137050.066.059416
GTATAAG105950.066.002646
GCTATAC55500.065.99385
GGTATAG126700.065.6851655
GGTATAC72250.065.558695
CATATAG98400.065.485335
GCTATAG86050.065.4522255
CCTATAC40150.065.434065
CTATAAC44550.065.4103856
CCTATAG53050.065.3488165
TTATAAG97150.065.283656
AGTATAG135350.065.18335
Produced by FastQC (version 0.11.5)