FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-5389-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-5389-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6624204
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC124080.18731307188003268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAA112550.067.471895
CTATAGG116650.067.4093556
CTATACG69450.067.28886
CTATACC63000.067.182076
TTATAGC99900.067.157066
CTTATAG93750.067.047935
GTATAGG213150.066.9550556
CTATAAC61500.066.829986
CGTATAG104900.066.757055
GGTATAG171000.066.6239245
TTATAGG187050.066.554716
GCTATAC66250.066.420685
GCTATAG113150.066.403095
GGTATAC99000.066.283625
CATATAG129350.066.280445
CCTATAC51750.066.105425
TTATAAG122800.066.055796
CGTATAA84850.066.0418555
GTATAAG139950.065.859176
GTATAGC101050.065.804316
Produced by FastQC (version 0.11.5)