FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-5389-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-5389-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104301
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC57170.18416384235935884No Hit
GAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCT35330.11380983996075124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG32500.067.159746
CTATAGG50650.066.9884646
TTATAGC49100.066.396046
GGTATAC44150.066.234365
TTATAAG55700.066.190126
CGTATAG46300.066.1806265
CTATACC28500.066.030596
GTATAGG95400.065.998176
GGTATAA54650.065.9897165
CTATAAC27150.065.9641346
GCTATAG49400.065.922025
CTTATAG41100.065.70275
GCTATAC32300.065.626295
TTATAGG82900.065.612056
GGTATAG77650.065.543455
CCTATAC24900.065.322285
CATATAG61450.065.233475
TATAGGG94000.065.232167
GTATAAG61100.065.206416
TATACGG55750.065.189617
Produced by FastQC (version 0.11.5)