FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-5389-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-5389-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5428308
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC105000.19343043909814991No Hit
GAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCT63410.11681356326870178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG54300.068.221386
GGTATAA97050.067.077255
CTATAGG92400.066.932146
GCTATAC56050.066.653055
TTATAGC83900.066.500236
CGTATAG77100.066.240395
GGTATAC79700.066.1861955
CTTATAC61750.066.0525
TTATAGG144250.065.9837656
CTATACC51600.065.825466
GCTATAG90550.065.7113345
CGTATAA65450.065.685075
GTATAGG165900.065.638276
GTATAAC56750.065.5227056
CTTATAG70600.065.402395
GTATAAG107950.065.391756
GGTATAG138500.065.338785
TATAAGG119550.065.3334357
TTATAAG100100.065.243116
CCTATAC46900.065.112725
Produced by FastQC (version 0.11.5)