FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-5389-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-5389-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5955946
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC118130.19833960885474786No Hit
GAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCT71440.11994736016746961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG100250.067.303846
CTATACG57100.067.076456
TTATAGC91800.066.907366
CGTATAG86300.066.875215
TTATAGG161100.066.24286
CCTATAC44750.066.203725
GTATAGG190100.066.201996
GGTATAC87000.065.9752965
CTTATAG80550.065.960555
CTATACC59300.065.944596
TTATAAG111450.065.906956
CTATAAC49050.065.890836
GGTATAA105050.065.860075
GCTATAC64550.065.783125
GTATAAG125150.065.763226
GCTATAG92900.065.625665
GGTATAG156800.065.5833055
TCTATAG88200.065.51395
TATAAGG138250.065.197957
TTTATAG129400.065.0629655
Produced by FastQC (version 0.11.5)