FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1771-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1771-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5614312
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC124750.2221999774861105No Hit
GAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCT69360.12354140632013326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG98250.067.676456
CTTATAG73900.067.3516855
GGTATAC80600.067.2643055
CTATACG51650.067.1106346
GCTATAC60550.067.0088045
CGTATAG80050.066.939965
TTATAGG152600.066.883476
GGTATAA101050.066.597335
GTATAGC84300.066.5940556
GTATAGG172600.066.590896
GGTATAG143050.066.433915
CTATACC53450.066.3557056
TTATAGC86250.066.264516
GCTATAG94800.066.154595
CGTATAC51050.065.912445
CTATAAG71050.065.868846
CATATAG115550.065.7775655
GTATAGA131700.065.732096
GTATAAG117150.065.476336
AGTATAG147500.065.4018865
Produced by FastQC (version 0.11.5)