FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1771-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1771-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5915864
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC139350.2355530823561867No Hit
TAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCCTG62430.10552980933976847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG159200.067.75636
CTATAGG104850.067.718296
CTATACG57500.067.4593356
CGTATAA72650.067.449845
GTATAGG179550.067.401426
GCTATAC61000.067.3730855
CGTATAG90150.067.237455
CTTATAG82400.067.109355
GGTATAA106800.066.940355
CTATACC56250.066.906476
GGTATAG149750.066.776815
GCTATAG100700.066.688385
GGTATAC89600.066.674265
CTATAAC55400.066.3546456
GTATAAG118900.066.2173846
ATTATAG124050.066.0059365
TTATAGC88800.065.975136
CCTATAC46150.065.93655
GTATAGC89450.065.9258356
TTTATAG123700.065.853395
Produced by FastQC (version 0.11.5)