FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1771-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1771-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693440
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC37080.1376678151360342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACC26050.067.811376
CTATAGG42000.067.461286
CTATACG28650.067.151696
GTATAGG80900.067.1493456
CGTATAA33500.066.931735
CTTATAG35000.066.861625
GGTATAG64200.066.854155
CGTATAG39800.066.795835
GCTATAC29400.066.6284265
TTATAGG69200.066.573066
TTATAGC36450.066.31316
GGTATAA49650.066.296295
CTATAAC26500.066.2638556
TATAGGG84450.066.104037
AGTATAG61300.065.908345
GTATAGC37000.065.894616
CTATAAG32350.065.634666
TCTATAG38000.065.541335
TATAACG47500.065.5376747
TTATAAG50650.065.470816
Produced by FastQC (version 0.11.5)