FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1771-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1771-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5777426
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC118150.20450283569188077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG93550.067.263826
GCTATAC57050.066.767695
CGTATAG80200.066.641255
TTATAGC84250.066.551496
TTATAGG147850.066.454536
CTTATAG75850.066.4050455
CTATAAC51750.066.373596
GGTATAC83450.066.309285
CTATACG55950.066.26746
GCTATAG91000.066.265895
GTATAGG168350.066.112146
CGTATAA69000.065.849765
CTATACC49350.065.703236
TATAGGG159600.065.701897
GGTATAG138350.065.582055
GGTATAA105350.065.573255
CATATAG117100.065.505225
TTATAAG101800.065.386066
GTATAAG114950.065.300266
CTTATAC61700.065.250575
Produced by FastQC (version 0.11.5)