FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1771-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1771-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5389061
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC95570.17734072781881668No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA61430.11399017379836672No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA57300.1063265010360803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG90200.068.174366
CTATACG51950.067.803596
CGTATAG79350.067.79825
TTATAGC75700.067.555436
GCTATAC55650.067.506865
GGTATAC76400.067.39435
CGTATAA69650.066.944955
GGTATAA99900.066.8769765
TTATAGG146550.066.8075946
GTATAGG160200.066.661196
GCTATAG89250.066.626885
GGTATAG129050.066.597195
CTATAAC51400.066.419486
CGTATAC53350.066.4176945
CTTATAC60300.066.36175
TTATAAG99000.066.2836466
CTTATAG70400.066.281445
GTATAAC62000.066.178226
GTATAAG107300.066.0462956
CTATACC48450.065.987426
Produced by FastQC (version 0.11.5)