FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1771-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1771-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6697492
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC115500.17245261360521222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG64500.067.192186
CGTATAG98150.067.106895
CTATAGG112850.066.889346
GTATAGG202100.066.842556
CGTATAA84150.066.632565
GCTATAC69100.066.363875
GGTATAC100200.066.292495
TTATAGG184550.066.280946
GGTATAA121650.066.248715
GGTATAG171550.066.246985
CTATAAC58000.066.218816
GCTATAG112250.066.1873865
TTATAGC105000.065.893736
CTTATAG94700.065.857995
TTATAAG123450.065.849446
GTATAAG138400.065.509766
CTATACC60350.065.495026
GTATAGA146550.065.25596
TATAGGG208400.065.2553567
GTATAGC102400.065.243996
Produced by FastQC (version 0.11.5)