FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1759-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1759-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3955568
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC72800.18404436480424557No Hit
GAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCT45340.11462323489319359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG39700.068.278956
GGTATAA72100.067.3818055
TTATAGG107900.067.0678946
CTATAGG69550.067.036096
CTTATAG53300.066.99995
CGTATAG58000.066.9376755
CCTATAC31450.066.838935
TTATAGC58850.066.684076
CTATACC38750.066.61326
GTATAGG119700.066.32966
GGTATAG101850.066.072345
GTATAAG80350.065.948116
GCTATAC40150.065.9456255
GTATAGC60800.065.810946
GGTATAC58100.065.678655
CTATAAC34150.065.6512456
AGTATAG104850.065.616285
TATAAGG90550.065.547797
GCTATAG65150.065.496955
GATATAC46850.065.473515
Produced by FastQC (version 0.11.5)