FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1759-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1759-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11937697
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC188770.15812932762491794No Hit
GAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCT172150.1442070442900335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG198350.067.716756
CTATACG116350.067.64156
TTATAGC180300.067.046356
CGTATAG172300.066.8906565
CCTATAC88250.066.626695
CTATACC110000.066.617526
GCTATAC123250.066.607245
CTTATAG159600.066.537155
TTATAGG331300.066.261096
GGTATAC181300.066.232525
GTATAGG380350.066.103096
GCTATAG192600.065.9424745
GTATAGC183900.065.7148366
CTATAAC105500.065.546776
GGTATAG310600.065.361395
CATATAG215850.065.2080465
TTTATAG259900.065.084165
GATATAC139850.064.928825
AGTATAG295050.064.905845
GTATAGA258700.064.8590246
Produced by FastQC (version 0.11.5)