FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1759-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1759-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8107169
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCT103660.12786214275291413No Hit
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC88910.10966836882270493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG86950.067.58756
CTATAGG137500.067.289866
TTATAGC120400.067.08486
CTATACC67650.067.065456
GTATAGG268750.066.850286
GCTATAG129700.066.670725
CCTATAC60350.066.657235
TTATAGG234300.066.646696
CGTATAG127350.066.555075
GCTATAC84500.066.4009555
TTATAAG152050.066.372186
GGTATAA154200.066.240735
GGTATAG213850.066.23165
TATAGGG276650.066.153947
GGTATAC119100.066.142915
CTTATAG106400.065.78585
GTATAAG170750.065.617996
TTTATAG186650.065.313275
CTATAAC72800.065.156086
GTATAAC84000.065.130466
Produced by FastQC (version 0.11.5)