FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1759-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1759-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4757121
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC118950.25004619390593597No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA49370.10378125761358603No Hit
TAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCCTG48110.10113259679541471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGC68900.068.026186
CTATAGG84200.067.795146
CTATACG46100.067.679076
GGTATAC69400.067.586485
CTATACC46350.067.540426
GGTATAG113600.067.3689045
GTATAGG140550.067.292456
CGTATAG67350.067.25475
TTATAGG132550.067.158126
CCTATAC35850.067.0282755
GCTATAC51550.066.901945
GCTATAG76000.066.64165
GGTATAA88050.066.61845
CTTATAG67800.066.601965
TTATAAG89300.066.351756
GTATAAG98250.066.324016
CTATAAC41400.066.1533666
TTTATAG101000.066.145835
TATAGGG143400.065.953667
GTATAGA110800.065.9461066
Produced by FastQC (version 0.11.5)