FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1759-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1759-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7811485
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC193790.24808343099935543No Hit
AATAAGTATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCC85350.10926219534441914No Hit
TAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCCTG85060.10889094711184877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG144550.068.118156
CTATACG73050.067.8503656
CGTATAG120400.067.574965
GCTATAG133850.067.553015
TTATAGC116250.067.5500956
CTTATAG103400.067.5215765
TTATAGG222500.067.489076
GGTATAA141300.067.407475
GGTATAC116800.067.082275
CTATAAC64450.067.080786
GTATAGG268450.067.012486
GCTATAC79350.066.871525
CTATACC74100.066.841726
GTATAAG168150.066.816126
GTATAGC125100.066.741766
GGTATAG212000.066.739855
GTATAGA194850.066.3848656
CCTATAC58000.066.3387455
CATATAG162450.066.275275
TTATAAG152900.066.251186
Produced by FastQC (version 0.11.5)