FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1759-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1759-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9404144
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC216890.2306323680283926No Hit
AATAAGTATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCC107160.11394976512482156No Hit
TAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCCTG105670.11236535722974891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG185750.068.503276
CTATACG91750.068.23776
GGTATAC148700.067.9770055
GTATAGG335100.067.949156
GGTATAA176050.067.8667455
CGTATAG150650.067.747195
TTATAGC140350.067.7352146
CTATACC87750.067.681196
GTATAAG213450.067.609936
TTATAGG289400.067.584346
GGTATAG264650.067.560695
GTATAGC151950.067.444166
CTTATAG131200.067.3669055
CCTATAC68900.067.363845
GCTATAG178600.067.2306755
GCTATAC95850.067.2159965
TTATAAG193800.066.975296
CTATAAC78900.066.8943946
CATATAG210700.066.848595
CGTATAA118100.066.813525
Produced by FastQC (version 0.11.5)