FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1169-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1169-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8492533
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC141690.16684068227936236No Hit
GAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCT98220.11565454028850991No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA91540.10778880694369984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG79700.067.585856
CGTATAG131050.067.205835
CTATAGG141900.067.145686
TTATAGC126450.067.023636
GGTATAA159000.066.765155
GTATAGG273350.066.6031956
CCTATAC63400.066.5895845
TTATAGG247450.066.563276
GGTATAC128250.066.409455
GGTATAG220650.066.3248145
CGTATAA102700.066.310735
CTATACC73000.066.2663046
GCTATAG141450.066.245765
TTATAAG161550.066.058496
GCTATAC86550.065.873215
GTATAAG180200.065.588366
CTTATAG117650.065.554875
GTATAGA199600.065.241796
TATAGGG280550.065.228997
TTTATAG188400.065.1650165
Produced by FastQC (version 0.11.5)