FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1169-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1169-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4178531
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC90200.21586533640650268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG70700.067.522966
CGTATAG65000.066.6647345
CTATACG38300.066.659546
CTATACC36800.066.525546
TTATAGG109600.066.245296
GGTATAA73900.066.11355
GCTATAG70800.066.044465
GTATAGG132500.066.04046
CTATAAC38350.065.9342656
CTTATAG57050.065.9008565
GGTATAG107200.065.803555
CGTATAA53050.065.595785
TTATAAG78400.065.575186
CCTATAC29850.065.494645
GGTATAC59450.065.475915
TATAGGG119950.065.4275747
TTATAGC63650.065.2764056
GTATAGC63900.065.239956
GCTATAC45300.065.160215
CATATAG85500.064.9974065
Produced by FastQC (version 0.11.5)