FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1169-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1169-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5836027
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC101330.1736283947966656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG57100.068.733386
CGTATAG99700.068.0645
CTATAGG113450.067.9544756
GTATAGG210100.067.777576
GGTATAA107300.067.6440055
GGTATAC95850.067.586485
CCTATAC44300.067.510825
GGTATAG164600.067.47225
TTATAGC85400.067.378136
TTATAAG113450.067.060336
CTATACC52950.066.919776
TTATAGG180150.066.910146
CTTATAG82750.066.872795
GTATAGC98800.066.665996
GCTATAG106100.066.496915
GTATAAG126050.066.462036
CGTATAA78000.066.460655
GCTATAC59400.066.4253165
TATAGGG220300.066.3697
GTATAAC60650.066.209766
Produced by FastQC (version 0.11.5)