FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1169-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1169-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5880298
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC125700.2137646765521067No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA63040.10720545115230555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGC89500.067.961536
CGTATAG88200.067.8930745
CTATAGG105300.067.662696
CTATACG59000.067.227616
CTATACC50300.066.825476
GTATAGG177550.066.7041556
GCTATAG99500.066.685985
TTATAGG156400.066.622416
CGTATAA75000.066.59745
GGTATAA107150.066.3970955
GGTATAC89300.066.351735
TTATAAG102850.066.34986
GGTATAG146250.066.296055
GCTATAC66450.066.112715
CTATAAC57800.066.021226
TTTATAG126550.065.9751745
CCTATAC46350.065.955655
CTTATAG84750.065.786825
GTATAAC62050.065.783316
GTATAAG121500.065.751626
Produced by FastQC (version 0.11.5)