FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1169-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1169-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10656475
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC194040.18208647793946872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG103750.067.867546
CGTATAG157550.067.7598655
CTATAGG185600.067.394736
TTATAGC155800.067.196356
CGTATAA135600.067.0171365
TTATAGG277400.066.944156
GGTATAA196050.066.906885
GTATAGG315450.066.886316
GCTATAG175550.066.8694155
CTATACC99550.066.830646
CTTATAG146150.066.5832065
CTATAAC101550.066.547786
GCTATAC116250.066.527655
GGTATAC156500.066.1805655
CCTATAC84800.066.162965
GGTATAG263150.066.129645
CTTATAC121500.066.04255
CTATAGC118200.065.962786
GTATAGC164400.065.936556
TTATAAG192000.065.804096
Produced by FastQC (version 0.11.5)