FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0130-1169-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0130-1169-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7090924
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA105020.14810481680525697No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA84320.11891257049151845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG76150.067.3829356
CTATAGG112200.066.5883266
CTATACC59300.066.475086
GTATAGG204700.066.452276
CGTATAG104950.066.2890855
CCTATAC61700.066.270325
GCTATAG112650.066.136035
GCTATAC80350.066.037325
GGTATAA132250.066.014395
TTATAGC103500.065.967376
GGTATAG171500.065.835345
GTATAAG139050.065.779486
GGTATAC107850.065.674175
CGTATAA95400.065.5921255
TATAGGG230850.065.590097
TTATAAG123300.065.4446646
TTATAGG189400.065.319796
CTATAAC70800.065.1136256
GTATAAC81400.065.056576
TATAGCG131750.064.936137
Produced by FastQC (version 0.11.5)