FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-p1107-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-p1107-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4580660
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC54370.11869468591862307No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA53480.11675173446621229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA64200.067.668475
CGTATAG67500.066.899415
GTATAGG112450.066.441826
CTATACG48800.066.301256
TTATAGC68800.066.296256
GGTATAA76750.066.265245
CTATAGG68150.066.216
CTATAAC48150.065.888686
CTTATAG57850.065.603375
GCTATAC56550.065.5032965
TTATAGG103900.065.479276
GGTATAC60000.065.4095845
CTATACC50850.065.072876
GCTATAG69450.065.071385
GGTATAG98300.064.9039465
TATAGGG107550.064.816747
CCTATAA48300.064.670195
GTATAGC71950.064.657756
TTATACG66800.064.56346
TTTATAG88950.064.451465
Produced by FastQC (version 0.11.5)