FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-p1107-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-p1107-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8076448
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC107430.13301639532626225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG82000.066.757876
CTTATAG105500.066.741095
GTATAGG212050.066.740526
TTATAGG193100.066.406746
CGTATAG119400.066.295225
CGTATAA114050.066.0314255
GGTATAG184500.065.8620765
CTATAGG124200.065.676346
GCTATAC99800.065.646145
GGTATAA148800.065.161685
TTATAGC121000.065.0716866
CGTATAC91350.064.864465
GCTATAG125900.064.7339555
GTATAGC133300.064.682796
TATAGGG202650.064.587887
CTTATAC100000.064.360565
CTATACC81850.064.273386
TATAAGG152300.064.168287
CTATAAC83500.064.1343466
GGTATAC110900.064.122115
Produced by FastQC (version 0.11.5)