FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-p1107-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-p1107-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13364918
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC205860.15403012573664876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG194400.066.573225
CGTATAA188450.066.447865
GTATAGG334850.066.122236
CTATACG137500.066.08926
CTTATAG174300.065.8419955
TTATAGG307850.065.581496
TTATAGC199350.065.323726
TATAGGG314050.065.0988547
GGTATAG300900.065.085225
CTATAGG200000.064.81416
GCTATAC163000.064.784285
GGTATAA230750.064.650445
GGTATAC177750.064.465655
CTATAAC139850.064.203326
GCTATAG211550.064.053185
GTATAGC207000.064.024826
TATAGGC245350.063.9244737
GTATAGA219350.063.9122626
CTTATAC166900.063.8782125
CCTATAA137600.063.6262785
Produced by FastQC (version 0.11.5)