FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-p1107-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-p1107-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6547214
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC113650.1735852837558082No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA68120.10404425454857594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG65750.067.5704046
GTATAGG157100.067.4929356
CTATAGG100650.066.8017966
CGTATAA93150.066.735435
CGTATAG98700.066.704385
GGTATAG136850.066.616455
CTTATAG88550.066.488645
TTATAGC95800.066.203486
GGTATAC89200.066.082575
TTATAGG151900.065.980476
GCTATAC79800.065.975925
GCTATAG95400.065.96825
GGTATAA114100.065.7341465
GTATAGA108750.065.557666
CTATAAC71700.065.32966
TATAGGG154350.065.179987
CCTATAC58350.065.049025
GTATAAC75950.065.036256
CTATACC71100.064.995286
ATTATAG120750.064.953175
Produced by FastQC (version 0.11.5)