FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-p1107-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-p1107-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9801708
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC175340.17888718986527655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG148100.066.459525
TTATAGG232150.066.0224846
CTATACG100150.066.008386
CGTATAA137800.065.944435
CTTATAG131300.065.9323655
GGTATAG224850.065.817035
GTATAGG264050.065.7696
TTATAGC144500.065.5978556
GCTATAC116300.065.443635
CTATAGG150350.065.441696
GGTATAC130100.065.196245
GGTATAA171750.064.9043055
CTATAAC100450.064.6969766
GCTATAG150400.064.629225
TATAGGG243150.064.358437
GTATAGA161850.064.141546
CTATAGC115450.064.1379556
GTTATAG209000.064.0639655
TATAGGC191150.064.062167
TTTATAG193250.063.963945
Produced by FastQC (version 0.11.5)