FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-p1107-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-p1107-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11820313
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC255160.21586568815901916No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA124430.10526794002832243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG157050.067.8687745
CTATACG121100.067.304996
CGTATAA172850.067.0685045
TTATAGC177900.066.8163766
GGTATAC162800.066.717955
CTATAGG183000.066.71296
GCTATAC147300.066.709015
TTATAGG281650.066.4973146
GTATAGG319950.066.409196
TTATACC146500.066.1898046
GGTATAG272050.066.1055
GCTATAG178350.066.0397645
CTATACC132400.065.9465566
CTATAGC135600.065.7059566
GGTATAA217550.065.653185
CTATAAC128000.065.644526
AGTATAG235450.065.535095
GTATAGA194600.065.504486
TTTATAG220750.065.367035
GTATAAC140400.065.353176
Produced by FastQC (version 0.11.5)