FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-ARR2-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-ARR2-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10617734
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA183620.1729370880830128No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC144430.1360271410076764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG103850.067.057886
GTATAGG264200.066.273996
CGTATAG154750.066.089395
CTATAGG154700.066.042926
GGTATAG229350.066.0349965
CGTATAA149450.065.928925
TTATAGC151000.065.73866
GGTATAC139700.065.698055
CTATAAC107900.065.6754466
GGTATAA183900.065.6369255
CCTATAC88550.065.53045
CTATACC104650.065.007796
GCTATAG164650.064.8772355
TATAGGG264650.064.81237
GCTATAC125450.064.629065
TTATAGG244200.064.554566
CGTATAC117150.064.281625
GTATAGC161450.064.256666
TATAGCG178650.064.079917
TATACGG172550.063.6492967
Produced by FastQC (version 0.11.5)