FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-ARR2-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-ARR2-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2950152
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC50570.17141489658837916No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA42800.14507727059487105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG28250.066.98316
CGTATAG42050.066.7105945
GTATAGG73250.066.4688346
GGTATAC41250.066.223715
GGTATAG64500.066.2128755
CTATAGG40800.066.011116
TTATAGC42250.065.898096
CGTATAA40550.065.641775
TTATAGG69350.065.465836
GCTATAC34950.064.950725
CTATAAC30200.064.85866
GGTATAA53850.064.8233265
TATAGCG50550.064.695927
CTATACC29800.064.672816
CTATAGC34400.064.4646
TATAGGG68800.064.4647
CCTATAC24200.064.462325
CTTATAG40750.064.461265
TATAGGC60650.064.129887
GTATAGC46200.063.973666
Produced by FastQC (version 0.11.5)