FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-ARR2-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-ARR2-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6433085
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC108130.16808420843187988No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA68130.10590564247169128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG68450.067.8663256
GTATAGG156350.066.941196
CGTATAA93150.066.732025
CTATAGG100700.066.592056
TTATAGC93050.066.390216
CCTATAC59800.065.983845
CTATACC68250.065.912546
GGTATAA110350.065.8723145
GGTATAG132300.065.757565
TTATAGG149100.065.738566
CTATAAC72550.065.573936
TATAGGG145150.065.54937
GGTATAC88700.065.386885
CTTATAG90100.065.263845
GCTATAG99100.065.23165
GCTATAC82600.065.218445
CGTATAG96200.065.1617365
CGTATAC70750.064.9678655
ATTATAG118250.064.8143165
CTTATAC84750.064.678365
Produced by FastQC (version 0.11.5)