FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-ARR2-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-ARR2-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6654949
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC166260.249829112138951No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA81540.12252535669319178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG68050.067.748536
GTATAGG165600.066.705926
CGTATAA100700.066.693635
CTATAGG100300.066.68066
GGTATAG141200.066.1190265
TTATAGC95850.066.090486
CGTATAG101850.066.077965
GGTATAC87950.065.782785
CTATAAC69350.065.6210866
GCTATAC85100.065.5607155
TTATAGG158300.065.2300956
GCTATAG103650.065.166675
CTATACC72300.065.120746
GGTATAA116500.065.094745
TATAGGG162050.065.035867
CCTATAC60050.065.0075455
TATAAGG122400.064.441847
CTTATAC86150.064.355635
CTATAGC73250.064.276186
TATAACG92250.064.231767
Produced by FastQC (version 0.11.5)