FastQCFastQC Report
Wed 20 Feb 2019
HWHMVBGX9_n01_ZmTARGET.0116-ARR2-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWHMVBGX9_n01_ZmTARGET.0116-ARR2-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4475653
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC97800.21851559984654756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG44900.067.239266
CTATAGG69800.066.858846
GCTATAC52000.066.400645
GTATAGG115550.066.030446
GCTATAG69650.065.998295
CGTATAA63100.065.8081745
CGTATAG72650.065.728775
GGTATAG98300.065.482135
TTATAGG107000.065.2255256
GGTATAA77300.065.214315
CTATACC46350.064.909336
CTATAAC46950.064.899456
TTATAGC64100.064.890686
GGTATAC61450.064.785655
TATAGGG112450.064.675367
CCTATAC38000.064.350525
TATAACG63750.064.257027
CGTATAC50350.064.1993945
CTATAGC50800.064.181626
TATAGCG72400.064.165777
Produced by FastQC (version 0.11.5)